Research on the application of the hottest biochip

Research on the application of biochip technology in packaging (3)

2.3.2 protein chip

the basic principle of protein chip and DNA chip is the same, but it uses the specificity of antibody and antigen binding, that is, immune reaction. Protein chip takes protein instead of DNA as the detection object, which is closer to the material level of life activities than DNA chip, so it has a more direct application prospect than DNA chip. As far as the human genome is concerned, getting the sequence is only the first piece. The more arduous task is to collect, sort out, retrieve and analyze the information of the proteins expressed in the sequence and their structure and function, so as to comprehensively understand the proteins that dominate the chemical reactions in the human body, the functions of these proteins and the relationship between them, as well as the principle that they cause diseases. As an efficient tool to detect the existence and movement of proteins, protein chip will play an increasingly important role

2.3.3 chip laboratory

chip laboratory is the highest stage of the development of biochip technology. It is a highly integrated portable biological analysis system integrating sample preparation, gene amplification, nucleic acid labeling and detection. The equipment, testing, detection and display required by it will be completed on one chip, so that the existing cumbersome and inaccurate biological analysis process can be automated, continuous and miniaturized. The cost is extremely low and the use is very convenient

2.4 gene chip production methods

gene chip production methods mainly include on-chip synthesis method and spot sample method

2.4.1 on-chip synthesis

the principle of on-chip synthesis (in situ synthesis) method is to polymerize directly at each site by organic synthesis according to the pre-designed lattice sequence to obtain the required probe molecules. After polymerization, the fabrication of chip base is over. Synthesis methods mainly include the following: light deprotection parallel synthesis method; Preparation technology of photosensitive biodegradable plastics; Bio based high molecular materials and their key monomer preparation technology; Low carbon high molecular material preparation technology aiming at frugal resin; Preparation technology of flame retardant and environmental friendly high molecular foam materials; Parallel synthesis of anti-corrosion layers such as high-value recycling technology of waste rubber, plastics, fabrics and other materials; Microfluidic channel on-chip synthesis; On chip synthesis of molecular seals; Spray printing synthesis method

2.4.2 spot sample method

the synthesis work in the spot sample method is completed by the traditional DNA solid-phase synthesis instrument, but after synthesis, a special automatic micro sample device is used to coat it on the nitrocellulose membrane, nylon membrane or glass slide with a relatively high density. The support should be subject to specific treatment in advance, such as coating with positively charged polylysine or aminosilane, so that it can firmly bind oligonucleotide molecules. This method is currently used by most small and medium-sized companies. Advantages: cheap equipment, simple technology, short development cycle and high flexibility. Disadvantages: low lattice density

3 application of biochip technology in packaging

as mentioned above, biochip has some inherent characteristics of silicon chip, which can collect, detect and process data. In the field of packaging, it can be used to detect the specific signals of packaging and commodities and record the information of intelligent packaging

the operation of plastic granulator covers an extremely wide range of fields. 3.1 detection of microorganisms in packaging

when studying the shelf life of packaging and the quality of packaged goods, microorganisms in packaging are an important detection target, and it is often necessary to detect their characteristics and content for some specific microorganisms. These data can be detected conveniently and quickly by using gene chip

gene probe methodology utilizes the characteristics of DNA denaturation and re annealing. To make a gene probe, the DNA sequence of the gene studied must be clear. This gene may be unique to a specific microbial species. In this case, this DNA sequence is conducive to the detection of that microbial object. This gene may even be common to all bacteria, making it possible to detect all known bacteria

through a method called colony hybridization or transfer, gene probes can also be used to detect specific gene sequences in bacterial colonies, which grow on plates with mixed bacterial populations. When carrying out colony hybridization, press a piece of filter membrane gently on the plate to make the cells of each bacterial colony adhere to the filter membrane. Dissolve the cells directly on the filter membrane, and the DNA is fixed on the filter membrane. Then probe and detect the filter membrane as described above. Through this procedure, only those colonies containing specific DNA sequences have radioactive signals. Because the original plate contains all complete bacteria, the plastic extruder industry will get out of the dilemma, so the required colonies can be identified and retained for further research

(to be continued)